Inhibitory effect of dipeptide peptidase inhibitors analogues on LPS-induced inflammatory response on microglia / 局解手术学杂志

Objective To study the inhibitory effect and mechanism of dipeptide peptidase inhibitors analogues on LPS -induced inflam-matory response on microglia .Methods Microglia cells were cultured ,isolated and purified from the neonatal Sprague-Dawley rats.Divided them into blank group ,negative control ,LPS group and medicine group ( parallel determination for 3 times each group ) after pharmacological preconditioning for 48 hours.The optimal concentration of microglia proliferation induced by LPS were measured by MTT assay .Observed the role of dipeptide peptidase inhibitors analogues on LPS-induced microglia in different concentrations .The interleukin1β( IL-1β) ,tumor necro-sis factor alpha ( TNF-α) were assayed by enzyme-linked immunorrbent assay ( ELISA ) .The expression of TLR-4 was detected by Western blotting and the expression of NF-κB was detected by RT-PCR.Results LPS induced the proliferation of microglia and significantly in-creased the release of inflammatory cytokines in LPS-stimulated primary microglia .Compared with the blank group ,dipeptide peptidase inhibi-tors analogues could inhibit this effect and the IC 50 values was 1.014 ×10 -2 mol/L to MG after pretreatment for 48 hours.Dipeptide peptidase inhibitors analogues could inhibit the release of TNF-αand IL-1 significantly(P<0.01),and it decreased the expression of TLR4 and NF-κB signif-icantly(P<0.01).Conclusion This research suggests that dipeptide peptidase inhibitors analogues restrain cell proliferation and inflammatory re-sponse of LPS-stimulated microglia,and the possible mechanism may be related to the inhibition of the expression of TLR-4 and NF-κB.

Discussion of How to Guarantee the Interests of Both Doctor and Patient after Implementation of Drug“Zero Profit” / 中国药房

OBJECTIVE:To promote the implementation of drug“zero profit”,and ensure the interests of both doctor and pa-tient. METHODS:Through introducing the related information of drug management and compensation mechanism of drug“zero profit”,the advices were put forward for the heavy financial burden,shortage of hospital income and medical accessibility,etc. RESULTS&CONCLUSIONS:Drug“zero profit”could promote the reform of public hospital. If both doctor and patient could ob-tain benefits,it is very important to strictly enforce compensation mechanism and increase the government investment. Meanwhile, medical service price should be appropriately adjusted to reflect technical value,knowledge value and risk value of medical staff;preparyment of the total amount should be propeled by project,disease,head and other payment methods to control unreasonable medical expenses growth;mechanical of the hospital operating service should be improved to consciously reduce the dependence on drug profits.

Optimization of Preparation Technology and In Vitro Dissolution Rate of Ranolazine Sustained Release Tablets / 中国药房

OBJECTIVE:To study the preparation method of ranolazine sustained release tablets and to optimize the preparation technology conditions for these preparations. METHODS: The preparation technology conditions were optimized by orthogonal design with the amounts of HPMCP, MC, PH101 and the hardness of ranolazine sustained release tablets as indexes. The optimized results were verified and the in vitro release of ranolazine sustained release tablets was determined. RESULTS: The optimum preparation technology conditions of ranolazine sustained release tablets were as follows: the amounts of HPMCP, MC, PH101 and the hardness were 150mg, 75mg, 30mg, and 10kg, respectively; and the in vitro release rates at 2, 6, and 12h were (29.33?1.05)%, (59.9?1.53)% and (95.60?1.31)%, respectively. CONCLUSION: The delayed release effect of ranolazine sustained release tablets coincided with the predictive value on the whole, and the preparation technology was reproducible. The in vitro accumulated drug release was in conformity with the requirements.

Brief Analysis on the"Reforming or Canceling of the Drug Price Addition Policy in State Hospitals" / 中国药房

OBJECTIVE:To explore the rational structure of hospital drug charge in state hospitals and the approaches to improve the reimbursement mechanism for state hospitals.METHODS:The detailed document about"reforming or cancelling the drug price addition policy in state hospitals"stated in the Proposals on Deepening Reform of Medical System were analyzed. RESULTS & CONCLUSIONS:"Reforming or cancelling the drug price addition policy in state hospitals"does not necessarily mean the simply cancelling of drug price addition policy in state hospitals,instead,it was achieved by establishing reasonable structure of hospital drug charge and improving the reimbursement mechanism for state hospitals guaranteed by complete operational approaches and supporting policy.